TFF1 Polyclonal antibody

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  WB analysis using 13734-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13734-1-AP (TFF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 13734-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (TFF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13734-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TFF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13734-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

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Tested Applications

• Positive WB detected in: MCF-7 cells, human kidney tissue, mouse stomach tisssue
• Positive IHC detected in: human stomach cancer tissue, human colon cancer tissue, human ovary tumor tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4541

  Product name: Recombinant human TFF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-84 aa of BC032811

  Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF

• Full Name: trefoil factor 1
• Calculated Molecular Weight: 84 aa, 9 kDa
• Observed Molecular Weight: 9-12 kDa
• GenBank Accession Number: BC032811
• Gene Symbol: TFF1
• Gene ID (NCBI): 7031
• RRID: AB_2202853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04155
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	IHC	JCI Insight; Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing.; Authors - Wei Guo
human	WB,IHC	Bone; Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.; Authors - Chiara Spadazzi ; KO Validated
human	WB,IHC	Biomed Pharmacother; Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.; Authors - Jie Yi ; KD Validated
human	WB	Aesthet Surg J; Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization.; Authors - Bijun Kang
human	IHC	BMC Med Genomics; Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.; Authors - Kim Hark Kyun HK