TFF1 Recombinant monoclonal antibody, PBS Only

• 

  FC experiment of MCF-7 using 83467-2-RR (same clone as 83467-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tff1 Recombinant Antibody (83467-2-RR, Clone:240424F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83467-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83467-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83467-2-RR against Human TFF1 were performed. The affinity constant is 0.296 nM.

TFF1 Uni-rAb^® Recombinant Antibody for FC (Intra), Indirect ELISA

Cat No. 83467-2-PBS

Clone No.240424F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Synonyms

240424F5, Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2

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Product Information

83467-2-PBS targets TFF1 in FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4541

  Product name: Recombinant human TFF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-84 aa of BC032811

  Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF

• Full Name: trefoil factor 1
• Calculated Molecular Weight: 84 aa, 9 kDa
• GenBank Accession Number: BC032811
• Gene Symbol: TFF1
• Gene ID (NCBI): 7031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04155
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.