TFF1 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00469-2

  Sandwich ELISA standard curve of MP00469-2, TFF1 Recombinant Matched Antibody Pair - PBS only. 83467-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4541. 83467-3-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL.

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  Sample test of MP00469-2

  Urine of eight individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 73.7 ng/mL with a range of 12.4 - 196.2 ng/mL.

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  Sample test of MP00469-2

  Serum of sixteen individual healthy human donors was measured. The TFF1 concentration of detected samples was determined to be 4.6 ng/mL with a range of 1.1 - 14.6 ng/mL.

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  Affinity and Kinetic Characterization of 83467-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83467-3-PBS against Human TFF1 was performed. The affinity constant is 2.27 nM.

View 3D epitope

TFF1 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83467-3-PBS

Clone No.240424H4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Breast cancer estrogen-inducible protein, HP1.A, pNR 2, PNR-2, Polypeptide P1.A

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Product Information

83467-3-PBS targets TFF1 as part of a matched antibody pair:

MP00469-2: 83467-1-PBS capture and 83467-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TFF1 fusion protein Ag4541
• Full Name: trefoil factor 1
• Calculated Molecular Weight: 84 aa, 9 kDa
• GenBank Accession Number: BC032811
• Gene Symbol: TFF1
• Gene ID (NCBI): 7031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04155
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.