Trefoil factor 3 Polyclonal antibody

• Featured Product
• KD/KO Validated

Trefoil factor 3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 23277-1-AP

Synonyms

HITF, hP1.B, Intestinal trefoil factor, ITF, Polypeptide P1.B, TFF3, TFI, Trefoil factor 3, trefoil factor 3 (intestinal)

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Validation Data Gallery

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

• 

  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 23277-1-AP is Trefoil factor 3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Trefoil factor 3 fusion protein Ag9543
• Full Name: trefoil factor 3 (intestinal)
• Calculated Molecular Weight: 80 aa, 9 kDa
• GenBank Accession Number: BC017859
• Gene Symbol: TFF3
• Gene ID (NCBI): 7033
• RRID: AB_2879245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Trefoil factor 3 (TFF3) belongs to the TFF-domain peptide family which consists of three small secreted proteins (TFF1, TFF2, TFF3) that are expressed by mucous-secreting epithelia. TFF3 is a major constituent in the goblet cells in small and large intestines, especially a typical secretary peptide of the normal human antral and pyloric gastric mucosa. TFF3 is essential in regulating cell migration and maintaining normal GI mucosal integrity. TFF3 has been reported to be overexpressed at the gene and the protein level in human neoplasms such as prostate, breast, and colon cancer. TFF3 is involved in tumor cell scattering, angiogenesis, and invasion.

Publications

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
	WB,IHC	Neoplasia; Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.; Authors - Wen-Cheng Chung
human	IHC	Rhinology; Expression of Trefoil factor family peptides in the nasal allergic mucosa.; Authors - Miyahara Nobuyuki N
human	WB	J Histotechnol; Trefoil factor 3 knock-down prevents autophagy-related gene 12 elevation in colon adenocarcinoma.; Authors - Zhuo Zhang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (06-21-2019)

There blot doesn't come out clean.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Trefoil factor 3 Antibody Western Blot, validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:23277-1-AP)