TFIP11 Monoclonal antibody, PBS Only

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  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66114-1-Ig (same clone as 66114-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66114-1-Ig (same clone as 66114-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

TFIP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66114-1-PBS

Clone No.1A1C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1A1C8, bK445C9.6, hNtr1, HSPC006, NTR1

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Product Information

66114-1-PBS targets TFIP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5821

  Product name: Recombinant human TFIP11 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 492-837 aa of BC033080

  Sequence: IVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQHMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSVKDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVPMNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK

• Full Name: tuftelin interacting protein 11
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC033080
• Gene Symbol: TFIP11
• Gene ID (NCBI): 24144
• RRID: AB_2881513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBB9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.