Validation Data Gallery
|Positive WB detected in
|K-562 cells, HL-60 cells, mouse liver tissue, PC-3 cells
|Positive IHC detected in
|human tonsillitis tissue, human brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10097-2-AP targets TFPT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|TFPT fusion protein Ag0131
|TCF3 (E2A) fusion partner (in childhood Leukemia)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.