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- KD/KO Validated
CD71 Polyclonal antibody
CD71 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (3)
WB, IP, IHC, IF, ELISA
Cat no : 10084-2-AP
|Positive WB detected in||HT-1080 cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 47 publications below|
|IHC||See 16 publications below|
|IF||See 4 publications below|
10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, chicken, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD71 fusion protein Ag0123|
|Full Name||transferrin receptor (p90, CD71)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC001188|
|Gene ID (NCBI)||7037|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
|Product Specific Protocols|
|WB protocol for CD71 antibody 10084-2-AP||Download protocol|
|IHC protocol for CD71 antibody 10084-2-AP||Download protocol|
|IF protocol for CD71 antibody 10084-2-AP||Download protocol|
|IP protocol for CD71 antibody 10084-2-AP||Download protocol|
|Click here to view our Standard Protocols|
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.
Cell Death Differ
Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis
Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes
Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models.
miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia.
Cell Chem Biol
3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sara (Verified Customer) (10-25-2020)
Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.