|Positive WB detected in||HT-1080 cells, K-562 cells, HeLa cells, MCF-7 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD71 fusion protein Ag0123|
|Full Name||transferrin receptor (p90, CD71)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC001188|
|Gene ID (NCBI)||7037|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
|Product Specific Protocols|
|WB protocol for CD71 antibody 10084-2-AP||Download protocol|
|IHC protocol for CD71 antibody 10084-2-AP||Download protocol|
|IF protocol for CD71 antibody 10084-2-AP||Download protocol|
|IP protocol for CD71 antibody 10084-2-AP||Download protocol|
|Click here to view our Standard Protocols|
A novel iron(II) phenanthroline complex exhibits anticancer activity against TFR1-overexpressing esophageal squamous cell carcinoma cells through ROS accumulation and DNA damage.
Iron homeostasis and its disruption in mouse lung in iron deficiency and overload.
Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure.
Oxid Med Cell Longev
Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.
Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.
J Anim Physiol Anim Nutr (Berl)
Tolerable upper intake level of iron damages the liver of weaned piglets.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sara (Verified Customer) (10-25-2020)
Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.