CD71 Polyclonal antibody

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  WB analysis using 10084-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10084-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10084-2-AP (CD71 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10084-2-AP

  IP result of anti-CD71 (IP:10084-2-AP, 4ug; Detection:10084-2-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10084-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10084-2-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10084-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of K-562 using 10084-2-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of K-562 using 10084-2-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (10084-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• Featured Product
• KD/KO Validated

CD71 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10084-2-AP

Publications(116)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment

Synonyms

p90, sTfR, T9, TFR, TFR1

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Tested Applications

• Positive WB detected in: HT-1080 cells, LoVo cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 95 publications below
• IHC: See 21 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10084-2-AP targets CD71 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, monkey, chicken, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD71 fusion protein Ag0123
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• RRID: AB_2240403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	IHC	Acta Neuropathol; Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.; Authors - Till S Zimmer
	WB,IHC	Cell Death Differ; Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis; Authors - Qing-Xin Ji
	WB,IF	J Nanobiotechnology; Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe2+ and boosting Fenton reaction; Authors - Xue Li
	WB	Acta Biomater; Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes; Authors - Wenqiang Qian
human	IHC	Cancer Res; Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models.; Authors - Fu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nu (Verified Customer) (10-24-2023)

Although there is a band close to 100 kd, there are other even more stronger non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: postmortem human frontal cortex

CD71 Antibody Western Blot validation (1:1000 dilution) in postmortem human frontal cortex (Cat no:10084-2-AP)

FH

Sara (Verified Customer) (10-25-2020)

Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 WB / 1:100 IF
• Cell Tissue Type: B cells from mouse spleen

CD71 Antibody Western Blot,Immunofluorescence validation (1:1000 WB / 1:100 IF dilution) in B cells from mouse spleen (Cat no:10084-2-AP)