CD71 Antibody

CD71 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 10084-2-AP

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR



Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10084-2-AP targets CD71 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human samples.

Reactivity human
Cited Species human, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD71 fusion protein Ag0123
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession number BC001188
Gene symbol TFRC
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 10084-2-APDownload protocol
IHC protocol for CD71 antibody 10084-2-APDownload protocol
IF protocol for CD71 antibody 10084-2-APDownload protocol
IP protocol for CD71 antibody 10084-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biochem Pharmacol

A novel iron(II) phenanthroline complex exhibits anticancer activity against TFR1-overexpressing esophageal squamous cell carcinoma cells through ROS accumulation and DNA damage.

Authors - Jiecheng Ye
mouseWB

Exp Physiol

Iron homeostasis and its disruption in mouse lung in iron deficiency and overload.

Authors - Gisela Giorgi
monkeyWB

Data Brief

Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure.

Authors - Michael A Pettiglio
humanWB

Biochem Biophys Res Commun

Myricetin ameliorates scopolamine-induced memory impairment in mice via inhibiting acetylcholinesterase and down-regulating brain iron.

Authors - Beiyun Wang
humanWB

Oncol Rep

FBW7 increases the chemosensitivity of pancreatic cancer cells to gemcitabine through upregulation of ENT1.

Authors - Qiangsheng Hu
humanIHC

Brain Res

Dysregulated iron metabolism in the choroid plexus in fragile X-associated tremor/ataxia syndrome.

Authors - Jeanelle Ariza