|Positive WB detected in||HT-1080 cells, K-562 cells, HeLa cells, MCF-7 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, monkey, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD71 fusion protein Ag0123|
|Full Name||transferrin receptor (p90, CD71)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC001188|
|Gene ID (NCBI)||7037|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
|Product Specific Protocols|
|WB protocol for CD71 antibody 10084-2-AP||Download protocol|
|IHC protocol for CD71 antibody 10084-2-AP||Download protocol|
|IF protocol for CD71 antibody 10084-2-AP||Download protocol|
|IP protocol for CD71 antibody 10084-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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Biochem Biophys Res Commun
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sara (Verified Customer) (10-25-2020)
Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.