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  • KD/KO Validated

CD71 Polyclonal antibody

CD71 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 10084-2-AP


CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Tested Applications

Positive WB detected inHT-1080 cells, K-562 cells, HeLa cells, MCF-7 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10084-2-AP targets CD71 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD71 fusion protein Ag0123
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001188
Gene symbol TFRC
Gene ID (NCBI) 7037
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.


Product Specific Protocols
WB protocol for CD71 antibody 10084-2-APDownload protocol
IHC protocol for CD71 antibody 10084-2-APDownload protocol
IF protocol for CD71 antibody 10084-2-APDownload protocol
IP protocol for CD71 antibody 10084-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Pharmacol

A novel iron(II) phenanthroline complex exhibits anticancer activity against TFR1-overexpressing esophageal squamous cell carcinoma cells through ROS accumulation and DNA damage.

Authors - Jiecheng Ye

Exp Physiol

Iron homeostasis and its disruption in mouse lung in iron deficiency and overload.

Authors - Gisela Giorgi

Data Brief

Liver metal levels and expression of genes related to iron homeostasis in rhesus monkeys after inhalational manganese exposure.

Authors - Michael A Pettiglio

Oxid Med Cell Longev

Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.

Authors - Saideng Lu

Acta Neuropathol

Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy.

Authors - Till S Zimmer

J Anim Physiol Anim Nutr (Berl)

Tolerable upper intake level of iron damages the liver of weaned piglets.

Authors - Haoxuan Ding


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sara (Verified Customer) (10-25-2020)

Really good signal in WB, it worked great, even though I loaded 1-2 µg of total cell lysate in some lanes. Not that good for IF, the signal was a bit fuzzy and all over the cell.

  • Applications: Western Blot,Immunofluorescence
  • Primary Antibody Dilution: 1:1000 WB / 1:100 IF
  • Cell Tissue Type: B cells from mouse spleen
CD71 Antibody Western Blot,Immunofluorescence validation (1:1000 WB / 1:100 IF dilution) in B cells from mouse spleen (Cat no:10084-2-AP)