Tested Applications
| Positive WB detected in | A549 cells, U2OS cells, mouse kidney tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28426-1-AP targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29126 Product name: Recombinant human TGFB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 73-278 aa of NM_001135599 Sequence: STRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPPFFPSENAIPPTFYRPYFRIVRFDVSAMEKNASNLVKAEFRVFRLQNPKARVPEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYTSGDQKTIKSTRKKNSGKT Predict reactive species |
| Full Name | transforming growth factor, beta 2 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa, 51 kDa |
| GenBank Accession Number | NM_001135599 |
| Gene Symbol | TGFB2 |
| Gene ID (NCBI) | 7042 |
| RRID | AB_2918162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61812 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| IHC protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| WB protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TGFB2/TGF-beta 2 Polyclonal antibody (catalog 28426-1-AP) has 12 citations across: Advanced Materials, Small, Frontiers in Microbiology, Frontiers in Cellular and Infection Microbiology, Frontiers in Pharmacology, Frontiers in Chemistry, Cancers, Life, Molecular Carcinogenesis, International Journal of General Medicine, Journal of Ocular Pharmacology and Therapeutics, and Heliyon. Applications cited include WB, IHC, and IF in human, mouse, rat, and rabbit samples. Research fields span tissue engineering/hydrogels, scar therapy, renal carcinoma, necrotizing enterocolitis, liver fibrosis, tendon injury, breast/metastasis, esophageal cancer, colon cancer, gastric cancer, ocular meshwork, and intervertebral disc degeneration.
| Species | Application | Title |
|---|---|---|
Adv Mater Multifunctional Injectable Hydrogel as a Biomimic Lens: Optical-Mechanical Restoration and Dynamic Postoperative Microenvironment Regulation. | ||
Small MiR-141-3p-Functionalized Exosomes Loaded in Dissolvable Microneedle Arrays for Hypertrophic Scar Treatment | ||
Front Microbiol Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma. | ||
Front Cell Infect Microbiol M2-type macrophage nanovesicles regulate the inflammatory response after necrotizing enterocolitis by inducing M1 to M2-like macrophage polarization. | ||
Front Pharmacol 2-Dodecyl-6-methoxycyclohexa-2,5-dien-1,4-dione alleviates liver fibrosis and improves intestinal flora and bile acid metabolism | ||
Front Chem ADSCs Promote Tenocyte Proliferation by Reducing the Methylation Level of lncRNA Morf4l1 in Tendon Injury. |
Reviews
What customers say
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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rebecca (Verified Customer) (08-14-2025) | Unfortunately, this antibody did not work in our hands. We attempted to use it for western blot on mouse anterior segment tissue (eyes injected with vector expressing TGFb2).
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