Tested Applications
| Positive WB detected in | A549 cells, U2OS cells, mouse kidney tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
28426-1-AP targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29126 Product name: Recombinant human TGFB2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 73-278 aa of NM_001135599 Sequence: STRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPPFFPSENAIPPTFYRPYFRIVRFDVSAMEKNASNLVKAEFRVFRLQNPKARVPEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYTSGDQKTIKSTRKKNSGKT Predict reactive species |
| Full Name | transforming growth factor, beta 2 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa, 51 kDa |
| GenBank Accession Number | NM_001135599 |
| Gene Symbol | TGFB2 |
| Gene ID (NCBI) | 7042 |
| RRID | AB_2918162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61812 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| IHC protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| WB protocol for TGFB2/TGF-beta 2 antibody 28426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Microbiol Characteristics of Gut Microbiota in Patients With Clear Cell Renal Cell Carcinoma. | ||
Front Chem ADSCs Promote Tenocyte Proliferation by Reducing the Methylation Level of lncRNA Morf4l1 in Tendon Injury. | ||
Life (Basel) MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion | ||
Cancers (Basel) Profiling the Adrenergic System in Breast Cancer and the Development of Metastasis | ||
J Ocul Pharmacol Ther Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function | ||
Small MiR-141-3p-Functionalized Exosomes Loaded in Dissolvable Microneedle Arrays for Hypertrophic Scar Treatment |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rebecca (Verified Customer) (08-14-2025) | Unfortunately, this antibody did not work in our hands. We attempted to use it for western blot on mouse anterior segment tissue (eyes injected with vector expressing TGFb2).
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