Tested Applications
| Positive WB detected in | 3T3-L1 cells, mouse ovary tissue, HeLa cells, PC-12 cells |
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18942-1-AP targets TGF Beta 3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13507 Product name: Recombinant human TGFB3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-309 aa of BC018503 Sequence: MKMHLQRALVVLALLNFATVSLSLSTCTTLDFGHIKKKRVEAIRGQILSKLRLTSPPEPTVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQENTESEYYAKEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNRTNLFRAEFRVLRVPNPSSKRNEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESNLGLEISIHCPCHTFQPNGDILENIHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNPHLILMMIPPHRLDNPGQGGQRKKRALDTNYCFR Predict reactive species |
| Full Name | transforming growth factor, beta 3 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC018503 |
| Gene Symbol | TGFB3 |
| Gene ID (NCBI) | 7043 |
| RRID | AB_2202206 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10600 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| IHC protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| WB protocol for TGF Beta 3 antibody 18942-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TGF Beta 3 Polyclonal antibody (Cat# 18942-1-AP) has 39 citations across journals including Cancer Cell, Nature Immunology, Cancer Research, EMBO Journal, Biomaterials, Acta Biomaterialia, Cell Death & Disease, and Scientific Reports. Research fields span oncology (pancreatic, gastric, renal, prostate, colorectal cancers), fibrosis (pulmonary, liver, tissue), wound healing, rheumatoid arthritis, chondrogenesis, cardiac hypertrophy, immunotherapy, and developmental biology. Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Cancer Cell Proteogenomic characterization of non-functional pancreatic neuroendocrine tumors unravels clinically relevant subgroups | ||
Nat Immunol Spatial patterning of fibroblast TGFβ signaling underlies treatment resistance in rheumatoid arthritis. | ||
Cancer Res SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C. | ||
J Nanobiotechnology Human adipose-derived mesenchymal stem cell-derived exosomes induce epithelial remodeling and anti-scar healing revealed by single-cell RNA sequencing | ||
J Exp Clin Cancer Res HOXA10 mediates epithelial-mesenchymal transition to promote gastric cancer metastasis partly via modulation of TGFB2/Smad/METTL3 signaling axis. | ||
Adv Sci (Weinh) Flexible Organic Photovoltaic-Powered Hydrogel Bioelectronic Dressing With Biomimetic Electrical Stimulation for Healing Infected Diabetic Wounds |
