TGF Beta 3 Polyclonal antibody

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  WB analysis using 18942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18942-1-AP (TGF-beta 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 18942-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18942-1-AP (TGF-beta 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 18942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18942-1-AP (TGF Beta 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 18942-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 18942-1-AP (TGF Beta 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse ovary using 18942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using TGF Beta 3 antibody (18942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse ovary using 18942-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using TGF Beta 3 antibody (18942-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TGF Beta 3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 18942-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

TGFB3, ARVD, Latency-associated peptide, TGF beta3, TGF-beta 3

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, mouse ovary tissue, HeLa cells, PC-12 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

18942-1-AP targets TGF Beta 3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13507

  Product name: Recombinant human TGFB3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-309 aa of BC018503

  Sequence: MKMHLQRALVVLALLNFATVSLSLSTCTTLDFGHIKKKRVEAIRGQILSKLRLTSPPEPTVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQENTESEYYAKEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNRTNLFRAEFRVLRVPNPSSKRNEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESNLGLEISIHCPCHTFQPNGDILENIHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNPHLILMMIPPHRLDNPGQGGQRKKRALDTNYCFR

• Full Name: transforming growth factor, beta 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC018503
• Gene Symbol: TGFB3
• Gene ID (NCBI): 7043
• RRID: AB_2202206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P10600
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Cancer Res; SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C.; Authors - Dan Liu
	IF	NPJ Regen Med; Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration.; Authors - Alvin Bacero Bello
human	WB	J Med Chem; Dithiocarbazate-Copper Complexes for Bioimaging and Treatment of Pancreatic Cancer.; Authors - Yi Gou
human	WB,IHC	Sci Rep; Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling.; Authors - Lu Wang
human		Bone; CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-β3 function.; Authors - Yuya Yoshioka
human	IHC	Sci Rep; Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint.; Authors - Yingjie Li