TGF Beta 3 Polyclonal antibody

TGF Beta 3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18942-1-AP

Synonyms

ARVD, TGF beta 3, TGF beta3, TGFB3, TGF-beta 3, TGF-β3



Tested Applications

Positive WB detected in3T3-L1 cells, mouse ovary tissue, HeLa cells, PC-12 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

18942-1-AP targets TGF Beta 3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGF Beta 3 fusion protein Ag13507
Full Name transforming growth factor, beta 3
Calculated molecular weight 48 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC018503
Gene symbol TGFB3
Gene ID (NCBI) 7043
RRIDAB_2202206
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TGF Beta 3 antibody 18942-1-APDownload protocol
IHC protocol for TGF Beta 3 antibody 18942-1-APDownload protocol
IF protocol for TGF Beta 3 antibody 18942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancer Res

SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C.

Authors - Dan Liu
IF

NPJ Regen Med

Matrilin3/TGFβ3 gelatin microparticles promote chondrogenesis, prevent hypertrophy, and induce paracrine release in MSC spheroid for disc regeneration.

Authors - Alvin Bacero Bello
humanWB

J Med Chem

Dithiocarbazate-Copper Complexes for Bioimaging and Treatment of Pancreatic Cancer.

Authors - Yi Gou
humanWB,IHC

Sci Rep

Exosomes secreted by human adipose mesenchymal stem cells promote scarless cutaneous repair by regulating extracellular matrix remodelling.

Authors - Lu Wang
human

Bone

CCN4/WISP-1 positively regulates chondrogenesis by controlling TGF-β3 function.

Authors - Yuya Yoshioka
humanIHC

Sci Rep

Transforming growth factor beta 3 involved in the pathogenesis of synovial chondromatosis of temporomandibular joint.

Authors - Yingjie Li