Tested Applications
| Positive WB detected in | A431 cells, Daudi cells, HL-60 cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11522-1-AP targets TGIF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2085 Product name: Recombinant human TGIF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC012816 Sequence: MSDSDLGEDEGLLSLAGKRKRRGNLPKESVKILRDWLYLHRYNAYPSEQEKLSLSGQTNLSVLQICNWFINARRRLLPDMLRKDGKDPNQFTISRRGGKASDVALPRGSSPSVLAVSVPAPTNVLSLSVCSMPLHSGQGEKPAAPFPRGELESPKPLVTPGSTLTLLTRAEAGSPTGGLFNTPPPTPPEQDKEDFSSFQLLVEVALQRAAEMELQKQQDPSLPLLHTPIPLVSENPQ Predict reactive species |
| Full Name | TGFB-induced factor homeobox 2 |
| Calculated Molecular Weight | 237 aa, 26 kDa |
| Observed Molecular Weight | 28 kDa and 30 kDa |
| GenBank Accession Number | BC012816 |
| Gene Symbol | TGIF2 |
| Gene ID (NCBI) | 60436 |
| RRID | AB_2202731 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZN2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transforming growth factor-beta-induced factor 2(TGIF2), is a member of a subfamily of transcription factor with a TALE(3-amino acid loop extension), an atypical homeodomain. It also contains a proline-rich region, which is a putative SH3 domain-binding site and suggested involve in transcriptional regulation. It is highly expressed in the heart, kidney, and testis, but low in the brain and prostate. Through binding directly to the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins, TGIF2 acts as a transcriptional repressor. The calculated molecular weight of TGIF2 is 26 kDa, but the phosphorylated TGIF2 is about 30-35 kDa protein. This antibody detected two forms of bands of about 28 and 30 kDa, and the upper band appears to result from increased phosphorylation (PMID: 11427533).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| IHC protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| WB protocol for TGIF2 antibody 11522-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TGIF2 antibody (Cat# 11522-1-AP) has 9 total citations spanning journals including Nature Chemical Biology, International Journal of Biological Sciences, Cell Reports, Frontiers in Immunology, Biochemical Journal, Pathology Research and Practice, Adipocyte, Brain Behavior, and Experimental & Therapeutic Medicine. Research fields cover EMT and pancreatic cancer, glioma biomarkers, pluripotency in embryonic stem cells, mitochondrial ribosome maturation, gastric cancer via TGFβ signaling, tRNA modification and mitophagy, neuronal apoptosis, and vascular injury in hypertension.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Smad2 Cooperating with TGIF2 Contributes to EMT and Cancer Stem Cells Properties in Pancreatic Cancer via Co-Targeting SOX2
| ||
Cell Rep Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells. | ||
Front Immunol TGIF2 is a potential biomarker for diagnosis and prognosis of glioma
| ||
Biochem J The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. | ||
Pathol Res Pract FBLN2 inhibits gastric cancer proliferation and metastasis via the TGFβ/TGIF2 pathway |
