Anti-Human TGM2 Rabbit Recombinant Antibody

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• Anti-Human TGM2 Rabbit Recombinant Antibody (98749-1-RR)

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  FC experiment of HeLa using 98749-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human TGM2 Rabbit RecAb (98749-1-RR, Clone:253540A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  FC experiment of HUVEC using 98749-1-RR

  1x10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human TGM2 Rabbit RecAb (98749-1-RR, Clone:253540A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

TGM2 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98749-1-RR

Clone No.253540A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Erythrocyte transglutaminase, TG(C), TG2, TGase 2, TGase C

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells, HUVEC cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98749-1-RR targets TGM2 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  unknow

• Full Name: transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Gene Symbol: TGM2
• Gene ID (NCBI): 7052
• ENSEMBL Gene ID: ENSG00000198959
• RRID: AB_3746316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21980
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Transglutaminase 2 (TGM2) is a ubiquitous and multifunctional calcium-dependent enzyme belonging to the transglutaminase family. It is best known for its canonical activity of catalyzing the cross-linking of proteins by forming stable ε-(γ-glutamyl)lysine isopeptide bonds, which contributes to extracellular matrix stabilization and wound healing. Beyond this, TGM2 exhibits GTPase activity, allowing it to function as a signaling G-protein in intracellular processes. It is implicated in a wide range of physiological functions, including cell adhesion, proliferation, and apoptosis, as well as pathological conditions such as celiac disease, fibrosis, neurodegenerative disorders, and cancer metastasis, where its dysregulated expression often contributes to disease progression.