Multi-rAb® TGN46 Multi-Recombinant antibody

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• Multi-rAb® TGN46 Multi-Recombinant antibody (RMX00075)

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  WB analysis using RMX00075

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00075 (TGN46 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using RMX00075

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00075 (TGN46 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using RMX00075

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00075 (TGN46 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using RMX00075

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (RMX00075) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

TGN46 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00075

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TGOLN2, hTGN46, hTGN48, hTGN51, TGN38

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00075 targets TGN46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: trans-golgi network protein 2
• Calculated Molecular Weight: 447 aa, 47 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC028219
• Gene Symbol: TGN46
• Gene ID (NCBI): 10618
• RRID: AB_3750124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: O43493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.