TH Monoclonal antibody, PBS Only

• 

  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 66334-1-PBS

Clone No.2H7B7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

tyrosine hydroxylase, 2H7B7, DYT14, DYT5b, EC:1.14.16.2

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Product Information

66334-1-PBS targets TH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23075

  Product name: Recombinant human TH protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-260 aa of BC104967

  Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

• Full Name: tyrosine hydroxylase
• Calculated Molecular Weight: 528 aa, 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC104967
• Gene Symbol: TH
• Gene ID (NCBI): 7054
• RRID: AB_2881714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P07101
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.