TH Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TH Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H7B7

Cat no : 66334-1-PBS

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Validation Data Gallery

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  WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66334-1-Ig (same clone as 66334-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 66334-1-Ig (same clone as 66334-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66334-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66334-1-PBS targets TH in WB, IF, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TH fusion protein Ag23075
• Full Name: tyrosine hydroxylase
• Calculated Molecular Weight: 528 aa, 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC104967
• Gene Symbol: TH
• Gene ID (NCBI): 7054
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.