Filter: AllWBIHCIF/ICC

WB analysis of A549 using 12909-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of CHO cells using 12909-1-AP
WB result of 12909-1-AP from Takashi Kobayashi.
WB analysis of HeLa using 12909-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12909-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12909-1-AP (THADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of rat ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of rat ovary using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 12909-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 12909-1-AP (THADA antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A549 using 12909-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using THADA antibody (12909-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

THADA Polyclonal antibody

Name: THADA Polyclonal antibody
Brand: Proteintech
SKU: 12909-1-AP

THADA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12909-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

tRNA (32-2'-O)-methyltransferase regulator THADA, Thyroid adenoma-associated protein, thyroid adenoma associated, KIAA1767, GITA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, CHO cells
Positive IHC detected in	human pancreas tissue, human testis tissue, mouse ovary tissue, rat ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12909-1-AP targets THADA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity	human, mouse, rat, hamster
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3881 Product name: Recombinant human THADA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-400 aa of BC025773 Sequence: TSSALSMGPFVPFIMRCGHSPVYHSREMAARALVPFVMIDHIPNTIRSLLSTLPSCTDQCFRQNHIHGTLLQVFHLLQAYSDSKHGTNSDFQHELTDITVCTKAKLWLAKRQNPCLVTRAVYIDILFLLTCCLNRSAKDNQPVLESLGFWEEVRGIISGSELITGFPWAFKVPGLPQYLQSLTRLAIAAVWAAAAKSGERETNVPISFSQLLESAFPEVRSLTLEALLEKFLAAASGLGEKGVPPLLCNMGEKFLLLAMKENHPECFCKILKILHCMDPGEWLPQTEHCVHLTPKEFLIWTMDIASNERSEIQSVALRLASKVISHHMQTCVENRELIAAEL Predict reactive species
Full Name	thyroid adenoma associated
Calculated Molecular Weight	220 kDa
Observed Molecular Weight	183 kDa
GenBank Accession Number	BC025773
Gene Symbol	THADA
Gene ID (NCBI)	63892
RRID	AB_10638448
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6YHU6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for THADA antibody 12909-1-AP	Download protocol
IHC protocol for THADA antibody 12909-1-AP	Download protocol
WB protocol for THADA antibody 12909-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IF,IP	J Immunother Cancer THADA drives Golgi residency and upregulation of PD-L1 in cancer cells and provides promising target for immunotherapy. Authors - Chushu Li KD Validated View Article