THAP11 Polyclonal antibody

• Featured Product
• KD/KO Validated

THAP11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23030-1-AP

Synonyms

CTG B43a, CTG B45d, HRIHFB2206, RONIN, THAP domain containing 11, THAP11

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Validation Data Gallery

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  WB analysis of Jurkat using 23030-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23030-1-AP (THAP11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23030-1-AP (THAP11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 23030-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 23030-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23030-1-AP (THAP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

23030-1-AP targets THAP11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THAP11 fusion protein Ag19342
• Full Name: THAP domain containing 11
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC012182
• Gene Symbol: THAP11
• Gene ID (NCBI): 57215
• RRID: AB_2879200
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biomed Res Int; THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways.; Authors - Jing Zhang ; KD Validated
mouse	WB,IF	iScience; Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei; Authors - Hui Rong Soon