Thrombospondin 3-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

Thrombospondin 3-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19727-1-AP

Synonyms

THBS3, thrombospondin 3, Thrombospondin 3-Specific, TSP3

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Validation Data Gallery

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  IHC staining of human liver using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 19727-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19727-1-AP (Thrombospondin 3-Specific antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human liver tissue, human colon tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

19727-1-AP targets Thrombospondin 3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: thrombospondin 3
• Calculated Molecular Weight: 104 kDa
• GenBank Accession Number: NM_007112
• Gene Symbol: THBS3
• Gene ID (NCBI): 7059
• RRID: AB_10695895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THBS3, also named as TSP3, belongs to the thrombospondin family. THBS3 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. THBS3 can bind to fibrinogen, fibronectin, laminin and type V collagen. The antibody is specific to THBS3.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.; Authors - Davy Vanhoutte
human	IF	Front Cell Dev Biol; Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.; Authors - Mansheng Li
mouse	WB,IF	Nat Commun; Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability.; Authors - Tobias G Schips ; KO Validated