|Positive WB detected in||HeLa cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
55178-1-AP targets THOC2 in WB, IP, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||THO complex 2|
|Calculated molecular weight||183 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||NM_020449|
|Gene ID (NCBI)||57187|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.
Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.
Hum Mol Genet
Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability.