Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55178-1-AP targets THOC2 in WB, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | THO complex 2 |
| Calculated Molecular Weight | 183 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | NM_020449 |
| Gene Symbol | THOC2 |
| Gene ID (NCBI) | 57187 |
| RRID | AB_2881283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NI27 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for THOC2 antibody 55178-1-AP | Download protocol |
| IP protocol for THOC2 antibody 55178-1-AP | Download protocol |
| WB protocol for THOC2 antibody 55178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The THOC2 Polyclonal antibody (Cat# 55178-1-AP) has 7 citations published in Molecular Cell, International Journal of Biological Sciences, International Journal of Biological Macromolecules, PLoS Biology, BMC Cancer, Viruses, and Human Molecular Genetics. Research fields span mRNA export/THO complex biology, hepatocellular carcinoma, somatic cell reprogramming, bladder cancer and autophagy, acute myeloid leukemia, viral nuclear export, and THOC6-related intellectual disability.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Targeting THOC2-Mediated mRNA Export Induces PARP Inhibitor Vulnerability in DNA Repair-Competent Hepatocellular Carcinoma. | ||
Int J Biol Macromol Targeting mRNA export complex macromolecules THO subunits (Thoc2 and Thoc5) for somatic cell reprograming | ||
PLoS Biol CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy. | ||
BMC Cancer Deciphering lactate/lactylation networks in AML: integrated scRNA-seq and transcriptomics reveal functions and prognostic model. | ||
Viruses Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.
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Reviews
What customers say
The single review for this product reports a positive experience. The user tested it on macrophages for Western Blot and stated it "worked well," giving it a 5-star rating and saying they highly recommend it. Overall, the feedback is favorable, with no issues or complaints noted.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonam (Verified Customer) (10-16-2025) | Worked well. highly recommend
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