Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14862-1-AP
WB result of THOC5 antibody (14862-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THOC5 transfected HEK-293 cells.
IP result of anti-THOC5 (IP:14862-1-AP, 4ug; Detection:14862-1-AP 1:5000) with HEK-293 cells lysate 920 ug.
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14862-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:3000-1:8000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
THOC5, also named as C22orf19, or KIAA0983, is a 683 amino acid protein, which belongs to the THOC5 family. THOC5 is ubiquitously expressed in many tissues. THOC5 localizes in the nucleus and acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA.THOC5 plays a role in cytokine-stimulated monocytic development and affects granulocyte/macrophage differentiation.
Various lysates were subjected to SDS PAGE followed by western blot with 14862-1-AP (THOC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14862-1-AP
WB result of THOC5 antibody (14862-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THOC5 transfected HEK-293 cells.
IHC Figures
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14862-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14862-1-AP (THOC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 14862-1-AP
IP result of anti-THOC5 (IP:14862-1-AP, 4ug; Detection:14862-1-AP 1:5000) with HEK-293 cells lysate 920 ug.
IF/ICC Figures
IF Staining of HepG2 using 14862-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using THOC5 antibody (14862-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
THOC5 Polyclonal antibody
Catalog Number
14862-1-AP
Citations
1
Dilutions
WB : 1:3000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
