THRAP3 Recombinant antibody

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  WB analysis using 85093-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85093-1-RR (THRAP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 85093-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85093-1-RR (THRAP3 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human ovary cancer using 85093-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85093-1-RR (THRAP3 antibody) at dilution of 1:500 (under 20x lens).

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  IF Staining of HeLa using 85093-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THRAP3 antibody (85093-1-RR, Clone: 242268B5 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

THRAP3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85093-1-RR

Clone No.242268B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242268B5, BCLAF1 and THRAP3 family member 2, BCLAF2, Thyroid hormone receptor-associated protein 3, Thyroid hormone receptor-associated protein complex 150 kDa component

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells, LNCaP cells, human brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85093-1-RR targets THRAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: thyroid hormone receptor associated protein 3
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 109-130 kDa
• GenBank Accession Number: NM_005119
• Gene Symbol: THRAP3
• Gene ID (NCBI): 9967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y2W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THRAP3, also named as TRAP150, plays a role in transcriptional coactivation.