Thyroid Hormone Receptor beta Recombinant monoclonal antibody, PBS Only

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  WB analysis of mouse pancreas using 83866-2-RR (same clone as 83866-2-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83866-2-RR (Thyroid Hormone Receptor beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83866-2-RR (same clone as 83866-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thyroid Hormone Receptor beta antibody (83866-2-RR, Clone: 240619E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83866-2-RR (same clone as 83866-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Thyroid Hormone Receptor beta Recombinant Antibody (83866-2-RR, Clone:240619E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83866-2-PBS in a different storage buffer formulation.

Thyroid Hormone Receptor beta Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83866-2-PBS

Clone No.240619E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

THRB, THR1, NR1A2, ERBA2, c-erbA-beta

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Product Information

83866-2-PBS targets Thyroid Hormone Receptor beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16276

  Product name: Recombinant human THRB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 112-461 aa of BC106929

  Sequence: DKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED

• Full Name: thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
• Calculated Molecular Weight: 476 aa, 54 kDa
• Observed Molecular Weight: 53 kDa and 54 kDa
• GenBank Accession Number: BC106929
• Gene Symbol: Thyroid Hormone Receptor beta
• Gene ID (NCBI): 7068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10828
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Thyroid hormone receptor beta (THRB), also known as ERBA2, NR1A2, and THR1, belongs to the nuclear hormone receptor family and NR1 subfamily. THRB is a nuclear hormone receptor that can act as a repressor or activator of transcription. Moreover, THRB is also a high-affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. THRB is the most abundant THR isoform in the liver but is also expressed in other tissues and mediates the effects of THs on hepatic carbohydrate and lipid metabolism (PMID: 34884910). THRB has 2 isoforms with the molecular mass of 53 and 54 kDa (refer to UniProt).