THRSP Polyclonal antibody

• Featured Product
• KD/KO Validated

THRSP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 13054-1-AP

Synonyms

S14, Spot 14 protein, SPOT14, THRSP

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13054-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13054-1-AP (THRSP Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13054-1-AP

  IP result of anti-THRSP (IP:13054-1-AP, 4ug; Detection:13054-1-AP 1:300) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 13054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13054-1-AP (THRSP antibody) at dilution of 1:200 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:100-1:600
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

13054-1-AP targets THRSP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THRSP fusion protein Ag3721
• Full Name: thyroid hormone responsive (SPOT14 homolog, rat)
• Calculated Molecular Weight: 146 aa, 17 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC031989
• Gene Symbol: THRSP
• Gene ID (NCBI): 7069
• RRID: AB_2877906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THRSP (Thyroid hormone-inducible hepatic protein)  is a protein that in humans is encoded by the THRSP gene. The protein encoded by this gene is similar to the gene product of S14, a rat gene whose expression is limited to liver and adipose tissue and is controlled by nutritional and hormonal factors. This gene has been shown to be expressed in liver and adipocytes, particularly in lipomatous modules. It is also found to be expressed in lipogenic breast cancers, which suggests a role in controlling tumor lipid metabolism (RefSeq). This antibody works well in IHC, WB and IF.

Publications

Species	Application	Title
human	WB	Mol Med; Insulin-inducible THRSP maintains mitochondrial function and regulates sphingolipid metabolism in human adipocytes.; Authors - Maria A Ahonen
human	WB	Sci Rep; Elevated serum S14 levels are associated with more severe liver steatosis by ultrasonography.; Authors - Wen-Ti Lin
mouse	WB	Mol Cell Endocrinol; MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.; Authors - Ni Zeng ; KD Validated
		Medicine (Baltimore); Serum Spot 14 concentration is negatively associated with thyroid-stimulating hormone level.; Authors - Yen-Ting Chen
		PLoS One; The serum level of a novel lipogenic protein Spot 14 was reduced in metabolic syndrome.; Authors - Yen-Ting Chen
mouse	IHC	Adv Sci (Weinh); Spatial Transcriptomic Study Reveals Heterogeneous Metabolic Adaptation and a Role of Pericentral PPARα/CAR/Ces2a Axis During Fasting in Mouse Liver; Authors - Shiguan Wang