THSD4 Polyclonal antibody

THSD4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20619-1-AP

Synonyms

ADAMTSL-6, THSD4

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Validation Data Gallery

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  IHC staining of human oesophagus cancer using 20619-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 20619-1-AP (THSD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 20619-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 20619-1-AP (THSD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 20619-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 20619-1-AP (THSD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 20619-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 20619-1-AP (THSD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 20619-1-AP is THSD4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THSD4 fusion protein Ag14682
• Full Name: thrombospondin, type I, domain containing 4
• Calculated Molecular Weight: 490 aa, 55 kDa
• GenBank Accession Number: BC008840
• Gene Symbol: THSD4
• Gene ID (NCBI): 79875
• RRID: AB_2878709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	ACS Biomater Sci Eng; Matrisome Provides a Supportive Microenvironment for Skin Functions of Diverse Species.; Authors - Binghui Liu