THUMPD3 Polyclonal antibody

THUMPD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19807-1-AP

Synonyms

tRNA (guanine(6)-N2)-methyltransferase THUMP3, THUMP domain-containing protein 3, THUMP domain containing 3, EC:2.1.1.256

---

Validation Data Gallery

• 

  WB analysis of HeLa using 19807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19807-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19807-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19807-1-AP (THUMPD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19807-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19807-1-AP (THUMPD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19807-1-AP

  Immunofluorescent analysis of Hela cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 19807-1-AP

  Immunofluorescent analysis of HepG2 cells, using THUMPD3 antibody 19807-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse kidney tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19807-1-AP targets THUMPD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THUMPD3 fusion protein Ag13833
• Full Name: THUMP domain containing 3
• Calculated Molecular Weight: 507 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC001622
• Gene Symbol: THUMPD3
• Gene ID (NCBI): 25917
• RRID: AB_10644140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THUMPD3, or THUMP domain containing 3, is a protein-coding gene that plays a significant role in tRNA methylation, a post-transcriptional modification that affects tRNA biology and is closely associated with human diseases. THUMPD3 is responsible for catalyzing the formation of N2-methylguanosine (m2G) at position 6 in a broad range of tRNA substrates containing the characteristic 3'-CCA terminus of mature tRNAs.