Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17649-1-AP targets TIAR in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11884 Product name: Recombinant human TIAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC030025 Sequence: MDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVYGNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIPPPNQAGYGMASYQTQ Predict reactive species |
| Full Name | TIA1 cytotoxic granule-associated RNA binding protein-like 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 38-43 kDa |
| GenBank Accession Number | BC030025 |
| Gene Symbol | TIAR |
| Gene ID (NCBI) | 7073 |
| RRID | AB_3669280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01085 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIAR, also named as TIA 1 related protein, is an RNA-binding protein which is associated with apoptosis. It has been shown that mouse with disrupted TIAR gene show high levels of embryonic lethality. TIAR plays an important role in cytoplasm and nucleus where it can control translation of some specific mRNAs and activate splicing of exons with weak 5-splice sites.. Besides, TIAR also has the 28 kDa and 50 kDa proteins for TIAR exists in multiple alternative splicing forms.(PMID: 12533540, 7533298, 22851315, 8176212)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TIAR antibody 17649-1-AP | Download protocol |
| WB protocol for TIAR antibody 17649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TIAR Polyclonal Antibody (Cat# 17649-1-AP) has 2 citations published in Cell Reports and International Journal of Molecular Sciences. The cited studies focus on RNA biology, covering m6A-mediated circRNA translocation to stress granules linked to cellular senescence and chemoresistance, as well as TIA1/TIAL1 involvement in alternative splicing regulation of SIRT1 pre-mRNA. Applications used include WB, RIP, IP, and IF in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep m6A-mediated circG3BP1 translocation to stress granules promotes nucleation and senescence-linked chemoresistance. | ||
Int J Mol Sci HuR and TIA1/TIAL1 Are Involved in Regulation of Alternative Splicing of SIRT1 Pre-mRNA.
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