TIAM2 Polyclonal antibody, PBS Only

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• TIAM2 Polyclonal antibody, PBS Only (26870-1-PBS)

• 

  WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 26870-1-AP (same clone as 26870-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26870-1-AP (TIAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 26870-1-AP (same clone as 26870-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIAM2 antibody (26870-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 26870-1-AP (same clone as 26870-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human TIAM2 (26870-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26870-1-PBS in a different storage buffer formulation.

TIAM2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 26870-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

26870-1-PBS targets TIAM2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25469

  Product name: Recombinant human TIAM2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1346-1701 aa of BC137275

  Sequence: KDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS

• Full Name: T-cell lymphoma invasion and metastasis 2
• Observed Molecular Weight: 190-200 kDa
• GenBank Accession Number: BC137275
• Gene Symbol: TIAM2
• Gene ID (NCBI): 26230
• RRID: AB_3085914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVF5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells.