TICAM2 Polyclonal antibody

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• TICAM2 Polyclonal antibody (19524-1-AP)

TICAM2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19524-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2

• 

  WB analysis of PC-3 using 19524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 19524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 19524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human testis using 19524-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human testis tissue, PC-3 cells
• Positive IHC detected in: mouse testis tissue, human prostate hyperplasia tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19524-1-AP targets TICAM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: toll-like receptor adaptor molecule 2
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_021649
• Gene Symbol: TICAM2
• Gene ID (NCBI): 353376
• RRID: AB_10791887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86XR7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32 kDa.

Publications

What published studies show

The TICAM2 Polyclonal antibody (Cat# 19524-1-AP) has 1 total citation. It appears in the Journal of Hazardous Materials, in a study examining genome-wide interactions with cadmium exposure in dysglycemia, covering populational effects and molecular insights. The research field encompasses environmental toxicology, cadmium exposure, and metabolic dysregulation, with the antibody applied in Western Blot for human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Hazard Mater; Genome-wide interactions with cadmium exposure in dysglycemia: Populational effects and molecular insights.; Authors - Qi Jiang