TICAM2 Polyclonal antibody

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  WB analysis of PC-3 using 19524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19524-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19524-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19524-1-AP (TICAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

TICAM2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19524-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2, TIR domain-containing adapter molecule 2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, human testis tissue, PC-3 cells
• Positive IHC detected in: mouse testis tissue, human prostate hyperplasia tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19524-1-AP targets TICAM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: toll-like receptor adaptor molecule 2
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: NM_021649
• Gene Symbol: TICAM2
• Gene ID (NCBI): 353376
• RRID: AB_10791887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86XR7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32 kDa.