Tie1 Polyclonal antibody

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• Tie1 Polyclonal antibody (19329-1-AP)

Tie1 Polyclonal Antibody for WB, ELISA

Cat No. 19329-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

JTK14, TIE, TIE1

• 

  WB analysis of K-562 using 19329-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 19329-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (Tie1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 19329-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19329-1-AP targets Tie1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4728

  Product name: Recombinant human TIE1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 849-1138 aa of BC038239

  Sequence: NFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA

• Full Name: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• Calculated Molecular Weight: 1138 aa, 125 kDa
• Observed Molecular Weight: 154 kDa
• GenBank Accession Number: BC038239
• Gene Symbol: TIE1
• Gene ID (NCBI): 7075
• RRID: AB_10863655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35590
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Tie1 Polyclonal antibody (Cat# 19329-1-AP) has a total of 2 citations. These appear in Nature (2025), studying bone marrow niches, stem-cell hierarchy, and immune tolerance in mouse, and Nature Communications (2024), exploring an engineered heart tissue model for fibrotic processes and therapeutic interventions in human. The associated research fields include stem cell biology, immunology, cardiac tissue engineering, and fibrosis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Nature; Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance; Authors - Kazuhiro Furuhashi
human	IF	Nat Commun; Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions; Authors - Pengcheng Yang