Tie1 Polyclonal antibody

Tie1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Cat no : 19329-1-AP

Synonyms

JTK14, TIE, TIE1



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, NIH/3T3 cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19329-1-AP is Tie1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Tie1 fusion protein Ag4728
Full Name tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Calculated Molecular Weight 1138 aa, 125 kDa
Observed Molecular Weight 154 kDa
GenBank Accession NumberBC038239
Gene Symbol TIE1
Gene ID (NCBI) 7075
RRIDAB_10863655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Tie1 antibody 19329-1-APDownload protocol
FC protocol for Tie1 antibody 19329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols