TIGAR Polyclonal antibody

• Featured Product
• KD/KO Validated

TIGAR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22136-1-AP

Synonyms

C12orf5, TIGAR

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Validation Data Gallery

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  WB analysis using 22136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22136-1-AP (TIGAR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 22136-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 22136-1-AP (TIGAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse colon tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

22136-1-AP targets TIGAR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIGAR fusion protein Ag17532
• Full Name: chromosome 12 open reading frame 5
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012340
• Gene Symbol: TIGAR
• Gene ID (NCBI): 57103
• RRID: AB_2879003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa.; Authors - Yogita Kanan
mouse	WB	Neurobiol Dis; Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.; Authors - Amanda R Burmeister
human	WB	Cell Death Dis; MiR-652-5p elevated glycolysis level by targeting TIGAR in T-cell acute lymphoblastic leukemia.; Authors - Shan Liu
mouse	WB	Biochem Biophys Res Commun; Cordycepin exhibits anti-fatigue effect via activating TIGAR/SIRT1/PGC-1α signaling pathway; Authors - Xiaoming Chai
human,mouse	WB	Phytother Res; Dual targeting of wild-type p53 and gut microbiota by Magnolol represses key metabolic process and kills CRC cells; Authors - Haixia Ji
human	WB	Cancer Sci; Impact of TP53-induced glycolysis and apoptosis regulator on malignant activity and resistance to ferroptosis in intrahepatic cholangiocarcinoma; Authors - Katsuya Toshida ; KO Validated