TIGD1 Polyclonal antibody

TIGD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13833-1-AP

Synonyms

EEYORE, TIGD1

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Validation Data Gallery

• 

  WB analysis of HepG2 using 13833-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13833-1-AP (TIGD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 13833-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13833-1-AP (TIGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13833-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13833-1-AP (TIGD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue, human cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13833-1-AP targets TIGD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIGD1 fusion protein Ag4757
• Full Name: tigger transposable element derived 1
• Calculated Molecular Weight: 591 aa, 67 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC035143
• Gene Symbol: TIGD1
• Gene ID (NCBI): 200765
• RRID: AB_10597114
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang
human	WB	Front Med (Lausanne); Bioinformatics analysis and experimental verification of TIGD1 in non-small cell lung cancer; Authors - Lingchun Xia ; KD Validated
human	WB,IHC	Lung Cancer; MiR-137 mediated high expression of TIGD1 promotes migration, invasion, and suppresses apoptosis of lung adenocarcinoma; Authors - Yiqun Wei