KIM-1/HAVCR1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of HuH-7 using 84600-5-RR (same clone as 84600-5-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 84600-5-RR (TIM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84600-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84600-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84600-5-RR against Human TIM-1 were performed. The affinity constant is 0.314 nM.

KIM-1/HAVCR1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84600-5-PBS

Clone No.242001D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

HAVCR1, KIM-1, TIM-1, 242001D1, CD365

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Product Information

84600-5-PBS targets KIM-1/HAVCR1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1442

  Product name: Recombinant Human TIM-1/KIM-1/HAVCR protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 21-295 aa of NM_001173393.3

  Sequence: SVKVGGEAGPSVTLPCHYSGAVTSMCWNRGSCSLFTCQNGIVWTNGTHVTYRKDTRYKLLGDLSRRDVSLTIENTAVSDSGVYCCRVEHRGWFNDMKITVSLEIVPPKVTTTPIVTTVPTVTTVRTSTTVPTTTTVPMTTVPTTTVPTTMSIPTTTTVLTTMTVSTTTSVPTTTSIPTTTSVPVTTTVSTFVPPMPLPRQNHEPVATSPSSPQPAETHPTTLQGAIRREPTSSPLYSYTTDGNDTVTESSDGLWNNNQTQLFLEHSLLTANTTKG

• Full Name: hepatitis A virus cellular receptor 1
• Calculated Molecular Weight: 39kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001173393.3
• Gene Symbol: HAVCR1
• Gene ID (NCBI): 26762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96D42
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TIM-1, also known as kidney injury molecule (KIM-1), was first identified as a marker of acute kidney injury. TIM-1 mRNA and protein dramatically increase after ischemic kidney injury while there are low levels in normal kidneys. In addition, TIM-1 is expressed on CD4+ T cells after activation and its expression was sustained preferentially in Th2 but not Th1 cells. However, TIM-1 is expressed on both Th1 and Th2 cells and TIM-1 blockade prolongs survival of fully MHC-mismatched cardiac transplants. TIM-4, expressed by macrophages, is the ligand for TIM-1, and TIM-1 - TIM-4 interactions regulate Th cell responses and modulate Th1/Th2 cytokine balance. Moreover, TIM-1 can regulate macrophage activation and alter the co-stimulatory properties of macrophages. (PMID: 20091883)