TIMELESS Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 67022-1-Ig (same clone as 67022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67022-1-Ig (same clone as 67022-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67022-1-Ig (same clone as 67022-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67022-1-Ig (same clone as 67022-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67022-1-Ig (same clone as 67022-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67022-1-Ig (same clone as 67022-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 67022-1-Ig (same clone as 67022-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67022-1-Ig (TIMELESS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67022-1-Ig (same clone as 67022-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human TIMELESS (67022-1-Ig, Clone:1B6G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67022-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51685-1

  Cytometric bead array sample test of MP51685-1, TIMELESS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67022-2-PBS. Detection antibody: 67022-1-PBS.

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  Cytometric bead array standard curve of MP51685-1

  Cytometric bead array standard curve of MP51685-1, TIMELESS Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67022-2-PBS. Detection antibody: 67022-1-PBS. Standard:Ag6283. Range: 0.313-40 ng/mL.

• Featured Product
• KD/KO Validated

TIMELESS Monoclonal Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 67022-1-PBS

Clone No.1B6G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

hTIM, Protein timeless homolog, TIM, TIM1, timeless homolog (Drosophila)

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Product Information

67022-1-PBS targets TIMELESS as part of a matched antibody pair:

MP51685-1: 67022-2-PBS capture and 67022-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6283

  Product name: Recombinant human TIMELESS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 869-1208 aa of BC050557

  Sequence: QMGLADSVKDFQRKGTHIVLWTGDQELELQRLFEEFRDSDDVLGHIMKNITAKRSRARIVDKLLALGLVAERRELYKKRQKKLASSILPNGAESLKDFCQEDLEEEENLPEEDSEEEEEGGSEAEQVQGSLVLSNENLGQSLHQEGFSILLLWLQNCLIRAADDREEDGCSQAVPLVPLTEENEEAMENEQFQQLLRKLGVRPPASGQETFWRIPAKLSPTQLRRAAASLSQPEEEQKLQPELQPKVPGEQGSDEEHCKEHRAQALRALLLAHKKKAGLASPEEEDAVGKEPLKAAPKKRQLLDSDEEQEEDEGRNRAPELGAPGIQKKKRYQIEDDEDD

• Full Name: timeless homolog (Drosophila)
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC050557
• Gene Symbol: TIMELESS
• Gene ID (NCBI): 8914
• RRID: AB_2882337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UNS1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TIMELESS1 (TIM1) gene encodes protein timeless homolog belonging to timeless family. TIM1 is localized in nucleus and regulated by cell cycle. It expresses high levels in S, G2 and M phases, and low in G0 and G1 phases. TIM1 is involved in the circadian rhythm autoregulatory loop and is a crucial component of the circadian core oscillator, which includes the CRY proteins, CLOCK, NPAS2, etc. It is also involved in cell survival after DNA damage or replication stress and reported to play important role in epithelial cell morphogenesis and formation of branching tubules.