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  • KD/KO Validated

TIMM50 Polyclonal antibody

TIMM50 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22229-1-AP

Synonyms

TIM50, TIM50L, TIMM50



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22229-1-AP targets TIMM50 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIMM50 fusion protein Ag17574
Full Name translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC121147
Gene symbol TIMM50
Gene ID (NCBI) 92609
RRIDAB_2879039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TIMM50 antibody 22229-1-APDownload protocol
IHC protocol for TIMM50 antibody 22229-1-APDownload protocol
IF protocol for TIMM50 antibody 22229-1-APDownload protocol
IP protocol for TIMM50 antibody 22229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell Rep

TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria

Authors - Shiori Akabane
humanWB

Elife

Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Authors - Maxence Le Vasseur
mouseRIP

Front Cell Dev Biol

The Novel Non-coding Transcriptional Regulator Gm18840 Drives Cardiomyocyte Apoptosis in Myocardial Infarction Post Ischemia/Reperfusion.

Authors - Changjun Luo
humanWB

Front Cell Dev Biol

Applying Sodium Carbonate Extraction Mass Spectrometry to Investigate Defects in the Mitochondrial Respiratory Chain.

Authors - David R L Robinson
humanWB,RIP

Cell Prolif

Long non-coding RNA LOC100133669 promotes cell proliferation in oesophageal squamous cell carcinoma.

Authors - Zhuzhu Guan
  • KD Validated
humanIF

Mol Cell Proteomics

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Authors - Laura F Fielden