Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22229-1-AP targets TIMM50 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17574 Product name: Recombinant human TIMM50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 190-456 aa of BC121147 Sequence: GNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP Predict reactive species |
| Full Name | translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 456 aa, 50 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC121147 |
| Gene Symbol | TIMM50 |
| Gene ID (NCBI) | 92609 |
| RRID | AB_2879039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q3ZCQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TIMM50 antibody 22229-1-AP | Download protocol |
| IHC protocol for TIMM50 antibody 22229-1-AP | Download protocol |
| IP protocol for TIMM50 antibody 22229-1-AP | Download protocol |
| WB protocol for TIMM50 antibody 22229-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TIMM50 polyclonal antibody (Cat# 22229-1-AP) has accumulated 20 citations across journals including Nature Metabolism, Molecular Cell, EMBO Journal, Cell Reports, Molecular Psychiatry, Journal of Cell Biology, Drug Resistance Updates, Frontiers in Cell and Developmental Biology, iScience, and eLife. Associated research fields encompass mitochondrial biology, protein import, mitophagy, respiratory chain integrity, the integrated stress response, Alzheimer's disease, cancer, and cardiomyocyte apoptosis.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Proteasomes suppress anticancer drug-induced cytotoxicity by inhibiting mitochondrial protein import and promoting ROS-BNIP3-mediated mitophagy | ||
Mol Psychiatry Integrated multi-omics analyses of synaptosomes revealed synapse-associated novel targets in Alzheimer's disease. | ||
EMBO J Stress-induced OMA1-mediated cleavage of AIFM1 suppresses cell growth by controlling mitochondrial OXPHOS activity. | ||
EMBO J Poldip2 promotes mtDNA elimination during Drosophila spermatogenesis to ensure maternal inheritance |
