Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11368-1-AP targets TIN2 in WB, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1922 Product name: Recombinant human TIN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-451 aa of BC005030 Sequence: LFEYLCQLEKALPTPQAQQLQDVLSWMQPGVSITSSLAWRQYGVDMGWLLPECSVTDSVNLAEPMEQNPPQQQRLALHNPLPKAKPGTHLPQGPSSRTHPEPLAGRHFNLAPLGRRRVQSQWASTRGGHKERPTVMLFPFRNLGSPTQVISKPESKEEHAIYTADLAMGTRAASTGKSKSPCQTLGGRALKENPVDLPATEQKENCLDCYMDPLRLSLLPPRARKPVCPPSLCSSVITIGDLVLDSDEEENGQGEGKESLENYQKTKFDTLIPTLCEYLPPSGHGAIPVSSCDCRDSSRPL Predict reactive species |
| Full Name | TERF1 (TRF1)-interacting nuclear factor 2 |
| Calculated Molecular Weight | 451 aa, 50 kDa |
| Observed Molecular Weight | 50 kDa, 40 kDa |
| GenBank Accession Number | BC005030 |
| Gene Symbol | TINF2 |
| Gene ID (NCBI) | 26277 |
| RRID | AB_2205100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSI4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIN2, also named as TINF2, is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TIN2 has some isoforms with MW 50 kDa (TIN2L), 40 kDa (TIN2S) and 13 kDa. This antibody detects TIN2L, TIN2S and the shelterin complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TIN2 antibody 11368-1-AP | Download protocol |
| WB protocol for TIN2 antibody 11368-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRF1 antibody (Cat# 11368-1-AP) has 8 total citations. These appear in Nature Communications, Science Advances, eBioMedicine, Cell Reports, Oncotarget, Cells, International Immunopharmacology, and Tissue & Cell. The associated research fields center on telomere biology, including telomeric nucleosome recognition, alternative lengthening of telomeres, telomere dysfunction, and paracrine senescence, with additional applications in cancer (BRCA1/2, gastric cancer), heart failure, and mitochondrial homeostasis.
| Species | Application | Title |
|---|---|---|
Nat Commun Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence | ||
EBioMedicine Modified hTERT treatment ameliorates pressure overload-induced heart failure. | ||
Oncotarget BRCA1/2 mutations perturb telomere biology: characterization of structural and functional abnormalities in vitro and in vivo. | ||
Reviews
What customers say
The single review rates the antibody 5 stars. It performed excellently for immunofluorescence staining of fixed cardiac-specific cells and 3D organoids using a 1:100 primary dilution with a 1:250–1:400 Alexa Fluor secondary at 488 or 567 nm. It also worked well for Western blot, with 20–50 µg/µl protein loading for human-derived tissue and 40 µg/µl for mouse-derived tissue, using overnight primary incubation and a 1:300 secondary. Overall, the antibody was described as highly effective for both ICC and WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Refik (Verified Customer) (08-21-2025) | Worked brilliantly for ICC staining of fixed cardiac specific cells and 3D organoid structures with 1:100 primary antibody and 1:250 - 1:400 Alexa Fluor secondary at 488 or 567 wavelength for detection . Worked well for WB with 20-50ug/ul of protein loading for human-derived tissue and 40ug/ul of mouse-derived tissue for overnight primary antibody incubation and in combination with 1:300 secondary antibody
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