TINAG-Specific Polyclonal antibody

TINAG-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19747-1-AP

Synonyms

TIN AG, TINAG

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Validation Data Gallery

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  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19747-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19747-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, human kidney tissue, human placenta tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19747-1-AP targets TINAG-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulointerstitial nephritis antigen
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_014464
• Gene Symbol: TINAG
• Gene ID (NCBI): 27283
• RRID: AB_10642433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.