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  • Western Blot (WB) analysis of human skeletal muscle tissue using TJAP1 Polyclonal antibody (17734-1-AP)

    WB analysis of human skeletal muscle using 17734-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17734-1-AP (TJAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TJAP1 Polyclonal antibody (17734-1-AP)

    WB analysis of HepG2 using 17734-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17734-1-AP (TJAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

TJAP1 Polyclonal antibody

TJAP1 Polyclonal Antibody for WB, ELISA

Cat No. 17734-1-AP
Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PILT, Tight junction-associated protein 1, TJAP 1, TJP4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17734-1-AP targets TJAP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TJAP1 fusion protein Ag12174 Predict reactive species
Full Name tight junction associated protein 1 (peripheral)
Calculated Molecular Weight 547 aa, 61 kDa
Observed Molecular Weight 86-95 kDa
GenBank Accession NumberBC064401
Gene Symbol TJAP1
Gene ID (NCBI) 93643
RRIDAB_2240742
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5JTD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TJAP1, also named as PILT and TJP4, is colocalized at the tight junctions. TJAP1 has some forms with MW 60 kDa (native) and 86-90 kDa (modified).

Protocols

Product Specific Protocols
WB protocol for TJAP1 antibody 17734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Boyan (Verified Customer) (08-27-2025)

very good for both WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: human