ZO-3 Polyclonal antibody

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  WB analysis of MCF-7 using 22116-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 22116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 22116-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-29 using 22116-1-AP

  IP result of anti-ZO-3 (IP:22116-1-AP, 4ug; Detection:22116-1-AP 1:1000) with HT-29 cells lysate 1760 ug.

ZO-3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 22116-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3

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Tested Applications

• Positive WB detected in: HT-29 cells, MCF-7 cells, T-47D cells
• Positive IP detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

22116-1-AP targets ZO-3 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17495

  Product name: Recombinant human TJP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 84-330 aa of BC108906

  Sequence: SMENATSAFAIQILKTCTKMANITVKRPRRIHLPATKASPSSPGRQDSDEDDGPQRVEEVDQGRGYDGDSSSGSGRSWDERSRRPRPGRRGRAGSHGRRSPGGGSEANGLALVSGFKRLPRQDVQMKPVKSVLVKRRDSEEFGVKLGSQIFIKHITDSGLAARHRGLQEGDLILQINGVSSQNLSLNDTRRLIEKSEGKLSLLVLRDRGQFLVNIPPAVSDSDSSPLEDISDLASELSQAPPSHIPP

• Full Name: tight junction protein 3 (zona occludens 3)
• Calculated Molecular Weight: 952 aa, 105 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC108906
• Gene Symbol: ZO-3
• Gene ID (NCBI): 27134
• RRID: AB_2878998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95049
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Free Radic Biol Med; Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.; Authors - Restituto Tocmo
pig	WB	Chem Biol Interact; Benzo[a]pyrene induces epithelial tight junction disruption and apoptosis via inhibiting the initiation of autophagy in intestinal porcine epithelial cells; Authors - Jun Li