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TK1 Recombinant monoclonal antibody, PBS Only (Detector) (88812-2-PBS)

TK1 Recombinant monoclonal antibody, PBS Only (Detector)

Name: TK1 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 88812-2-PBS-100UG

TK1 Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 88812-2-PBS

Clone No. 261632C8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

EC:2.7.1.21, TK 1, TK 2, Tk1a, Tk1b

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHCCytometric bead array sample testCytometric bead arrayAffinity

WB analysis using 88812-2-RR (same clone as 88812-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 88812-2-RR (TK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88812-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 88812-2-RR (same clone as 88812-2-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88812-2-RR (TK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88812-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 88812-2-RR (same clone as 88812-2-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 88812-2-RR (TK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 88812-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP03672-1
The mean TK1 concentration was determined to be 45.2 ng/mL in SK-BR-3 cell extract based on a 1.2 mg/mL extract load, SH-SY5Y cell extract were served as a negative control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP03672-1
Cytometric bead array standard curve of MP03672-1, TK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88812-3-PBS. Detection antibody: 88812-2-PBS. Standard: Ag8249. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity of 88812-2-RR
Biolayer interferometry (BLI) kinetic assays of 88812-2-RR against Human TK1 were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

88812-2-PBS targets TK1 as part of a matched antibody pair:

MP03672-1: 88812-3-PBS capture and 88812-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag8249 Product name: Recombinant human TK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC007986 Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN Predict reactive species
Full Name	thymidine kinase 1, soluble
Calculated Molecular Weight	25 kDa
Observed Molecular Weight	25-30 kDa
GenBank Accession Number	BC007986
Gene Symbol	TK1
Gene ID (NCBI)	7083
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P04183
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).