TLE3 Monoclonal antibody

TLE3 Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D11A8

Cat no : 66083-1-Ig

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

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Validation Data Gallery

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  WB analysis of HEK-293 using 66083-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66083-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66083-1-Ig (TLE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 66083-1-Ig

  IP Result of anti-TLE3 (IP:66083-1-Ig, 5ug; Detection:66083-1-Ig 1:500) with HEK-293 cells lysate 1480ug.

• 

  IHC staining of human breast cancer using 66083-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 66083-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66083-1-Ig (TLE3 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: 293 cells, HEK-
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66083-1-Ig is TLE3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag17906
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated molecular weight: 772 aa, 83 kDa
• Observed molecular weight: 90 kDa
• GenBank accession number: BC015729
• Gene symbol: TLE3
• Gene ID (NCBI): 7090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )