TLE3 Monoclonal antibody

TLE3 Monoclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D11A8

Cat no : 66083-1-Ig

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3



Tested Applications

Positive WB detected in293 cells, HEK-
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66083-1-Ig is TLE3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TLE3 fusion protein Ag17906
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight 772 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC015729
Gene symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

Protocols

Product Specific Protocols
WB protocol for TLE3 antibody 66083-1-IgDownload protocol
IHC protocol for TLE3 antibody 66083-1-IgDownload protocol
IP protocol for TLE3 antibody 66083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols