TLE3 Monoclonal antibody
TLE3 Monoclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IP, IHC,ELISA
Conjugate
Unconjugated
CloneNo.
1D11A8
Cat no : 66083-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | 293 cells, HEK- |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 66083-1-Ig is TLE3 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TLE3 fusion protein Ag17906 |
Full Name | transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) |
Calculated molecular weight | 772 aa, 83 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC015729 |
Gene symbol | TLE3 |
Gene ID (NCBI) | 7090 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )
Protocols
Product Specific Protocols | |
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WB protocol for TLE3 antibody 66083-1-Ig | Download protocol |
IHC protocol for TLE3 antibody 66083-1-Ig | Download protocol |
IP protocol for TLE3 antibody 66083-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |