TLK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TLK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 13979-1-AP

Synonyms

TLK 2, Serine/threonine-protein kinase tousled-like 2, PKU-alpha, PKU ALPHA, HsHPK

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Validation Data Gallery

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  WB analysis of MCF-7 using 13979-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13979-1-AP

  WB result of TLK2 antibody (13979-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TLK2 transfected HeLa cells.

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  WB analysis of HeLa using 13979-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13979-1-AP (TLK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13979-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13979-1-AP (TLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13979-1-AP (TLK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13979-1-AP targets TLK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLK2 fusion protein Ag5022
• Full Name: tousled-like kinase 2
• Calculated Molecular Weight: 772 aa, 88 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC044925
• Gene Symbol: TLK2
• Gene ID (NCBI): 11011
• RRID: AB_2203887
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tousled-like kinases (TLKs) are a family of serine/threonine kinases located in the nucleus that promote S-phase chromatin assembly and mitotic chromosome segregation. There are two genes in the TLK family-TLK1 and TLK2 (PMID: 14561777). Mutation in TLK2 are associated with an elevated risk of breast cancer (PMID: 27489360). Tousled-like kinase 2 (TLK2) is a key factor in glioblastoma that modulates SRC signaling, thereby driving tumor malignancy. TLK2 is commonly upregulated in glioblastoma, and such upregulation was associated with poor patient outcomes. TLK2 overexpression induced cell growth, migration, invasion, and epithelialmesenchymal transition, and cell cycle arrest, while TLK2 knockdown had the opposite effect (PMID: 30207834). It can detect 3 isoforms of 88, 85 and 82 kDa.

Publications

Species	Application	Title
human	WB	Cancer Gene Ther; Targeting TLK2 inhibits the progression of gastric cancer by reprogramming amino acid metabolism through the mTOR/ASNS axis; Authors - Mingliang Wang ; KD Validated
human	IHC	Front Genet; Overexpression of tousled-like kinase 2 predicts poor prognosis in HBV-related hepatocellular carcinoma patients after radical resection; Authors - Bang Liu
human	WB	Transl Cancer Res; TLK2 promotes progression of hepatocellular carcinoma through Wnt/β-catenin signaling; Authors - Ting He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A selective and sensitiv antibody. KD validated

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T and HCT116