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Anti-Human TLR2 Rabbit Recombinant Antibody, PBS Only (98376-5-PBS)

Anti-Human TLR2 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human TLR2 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98376-5-PBS-1MG

TLR2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98376-5-PBS

Clone No.251864G3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

CD282, TIL4, toll like receptor 2, Toll/interleukin-1 receptor-like protein 4, Toll-like receptor 2

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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FC experiment of human PBMCs using 98376-5-RR (same clone as 98376-5-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98376-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98376-5-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 98376-5-RR (same clone as 98376-5-PBS)
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98376-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR2 Rabbit RecAb (98376-5-RR, Clone: 251864G3) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98376-5-PBS in a different storage buffer formulation.
Affinity of 98376-5-RR
Biolayer interferometry (BLl) kinetic assays of 98376-5-RR against Human TLR2 were performed. The affinity constant is 89.4 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98376-5-PBS targets TLR2 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	DNA Predict reactive species
Full Name	toll-like receptor 2
Calculated Molecular Weight	784 aa, 90 kDa
GenBank Accession Number	BC033756
Gene Symbol	TLR2
Gene ID (NCBI)	7097
RRID	AB_3746022
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O60603
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TLR2, also known as CD282, is a Toll-like receptor (TLR) family member. TLR receptors are type I integral transmembrane glycoproteins that play a fundamental role in pathogen recognition and activation of innate immunity. TLR2 is expressed by immune cells including monocytes/macrophages, polymorphonuclear leukocytes (PMLs), and dendritic cells, and is also expressed by some no-immune cells including endothelial and epithelial cells (PMID: 21428911; 22566960). TLR2 forms heterodimers with TLR1 and TLR6, which is the initial step in a cascade of events leading to significant innate immune responses, development of adaptive immunity to pathogens, and protection from immune sequelae related to infection with these pathogens (PMID: 22566960).