TLR3 Polyclonal antibody

TLR3 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 17766-1-AP

Synonyms

CD283, TLR3, toll like receptor 3



Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17766-1-AP is TLR3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR3 fusion protein Ag12171
Full Name toll-like receptor 3
Calculated molecular weight 904 aa, 104 kDa
GenBank accession numberBC094737
Gene symbol TLR3
Gene ID (NCBI) 7098
RRIDAB_2878436
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR3 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA. Upon recognition of dsRNA, TLR3 transmits signals via the adaptor protein TICAM-1/TRIF, leading to the induction of type I IFN, cytokine/chemokine production and dendritic cell (DC) maturation (PMID: 18262679).

Protocols

Product Specific Protocols
IHC protocol for TLR3 antibody 17766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

J Mater Chem B

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating

Authors - Bin Yan
human

J Cell Mol Med

Down-regulation of miR-10a-5p in synoviocytes contributes to TBX5-controlled joint inflammation.

Authors - Nazim Hussain
mouse

Phytomedicine

Wu-Mei-Wan prevents high-fat diet-induced obesity by reducing white adipose tissue and enhancing brown adipose tissue function.

Authors - Fan Wu
human

Front Cell Infect Microbiol

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Authors - Matthew Charman
human

RNA

MiR-146a down-regulates inflammatory response by targeting TLR3 and TRAF6 in Coxsackievirus B infection.

Authors - Yanru Fei
mouse

J Appl Toxicol

Intratracheal instillation of graphene oxide decreases anti-virus responses and lipid contents via suppressing Toll-like receptor 3 in mouse livers.

Authors - Xiaomin Tang