Anti-Human TLR4/CD284 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone:242067C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone: 242067C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

TLR4/CD284 Recombinant Antibody for FC

Cat No. 98327-1-RR

Clone No.242067C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TLR4, 242067C1, ARMD10, CD284, hToll

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98327-1-RR targets TLR4/CD284 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: toll-like receptor 4
• Calculated Molecular Weight: 96 kDa
• GenBank Accession Number: NM_138554
• Gene Symbol: TLR4
• Gene ID (NCBI): 7099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00206
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.