Anti-Human TLR4/CD284 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone: 242067C1) and PE-Conjugated Donkey Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were co-stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone:242067C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone: 242067C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98327-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TLR4/CD284 Rabbit RecAb (98327-1-RR, Clone:242067C1) (red) and PE-Conjugated Donkey Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

TLR4/CD284 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98327-1-RR

Clone No.242067C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TLR4, TOLL, hToll, CD284, ARMD10

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98327-1-RR targets TLR4/CD284 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: toll-like receptor 4
• Calculated Molecular Weight: 96 kDa
• GenBank Accession Number: NM_138554
• Gene Symbol: TLR4
• Gene ID (NCBI): 7099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00206
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.