TLR5 Polyclonal antibody

TLR5 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19810-1-AP

Synonyms

SLEB1, TIL3, TLR5, toll like receptor 5



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human tonsillitis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19810-1-AP targets TLR5 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR5 fusion protein Ag13840
Full Name toll-like receptor 5
Calculated molecular weight 858 aa, 98 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC109119
Gene symbol TLR5
Gene ID (NCBI) 7100
RRIDAB_10699876
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Protocols

Product Specific Protocols
IHC protocol for TLR5 antibody 19810-1-APDownload protocol
IP protocol for TLR5 antibody 19810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Prussian blue nanozyme-mediated nanoscavenger ameliorates acute pancreatitis via inhibiting TLRs/NF-κB signaling pathway.

Authors - Xue Xie
mouseFC

Int J Mol Sci

Myeloid-Derived Suppressor Cells Gain Suppressive Function during Neonatal Bacterial Sepsis.

Authors - Jordan K Vance
ratIHC, IF

Int J Mol Sci

Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling.

Authors - Ching Chang
humanIHC

EBioMedicine

Roseburia intestinalis stimulates TLR5-dependent intestinal immunity against Crohn's disease

Authors - Zhaohua Shen
humanIHC

Oncotarget

Toll-like receptor 5 gene polymorphism is associated with breast cancer susceptibility.

Authors - Chen Shuang
humanWB

Cells

Fibronectin Functions as a Selective Agonist for Distinct Toll-like Receptors in Triple-Negative Breast Cancer.

Authors - Anthony Ambesi