TLR5 Antibody

TLR5 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19810-1-AP

Synonyms

SLEB1, TIL3, TLR5, toll like receptor 5



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 19810-1-AP immunogen is Fusion Protein TLR5 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLR5 fusion protein Ag13840
Full Name toll-like receptor 5
Calculated molecular weight 858aa,98 kDa
Observed molecular weight 97 kDa
GenBank accession number BC109119
Gene symbol TLR5
Gene ID (NCBI) 7100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR5 belongs to the Toll-like receptor family which are important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR5 is known to recognize bacterial flagellin from invading mobile bacteria. TLR5 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Mutations in TLR5 gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.

Protocols

Product Specific Protocols
WB protocol for TLR5 antibody 19810-1-APDownload protocol
IHC protocol for TLR5 antibody 19810-1-APDownload protocol
IP protocol for TLR5 antibody 19810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Oncol Lett

Novel recombinant protein FlaA N/C increases tumor radiosensitivity via NF-κB signaling in murine breast cancer cells.

Authors - Ying Xu
mouseIF

Radiat Res

Novel Recombinant Protein FlaA N/C Protects against Radiation Injury via NF-κB Signaling.

Authors - Ying Xu
humanIHC

Oncotarget

Toll-like receptor 5 gene polymorphism is associated with breast cancer susceptibility.

Authors - Chen Shuang