TM9SF4 Polyclonal antibody

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  WB analysis using 25595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25595-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25595-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 25595-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25595-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25595-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

KIAA0255, TM9SF4

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Tested Applications

• Positive WB detected in: A375 cells, A431 cells, HepG2 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22335

  Product name: Recombinant human TM9SF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 25-244 aa of BC021107

  Sequence: IKAVKLTSSRTQLPYEYYSLPFCQPSKITYKAENLGEVLRGDRIVNTPFQVLMNSEKKCEVLCSQSNKPVTLTVEQSRLVAERITEDYYVHLIADNLPVATRLELYSNRDSDDKKKEKDVQFEHGYRLGFTDVNKIYLHNHLSFILYYHREDMEEDQEHTYRVVRFEVIPQSIRLEDLKADEKSSCTLPEGTNSSPQEIDPTKENQLYFTYSVHWEESDI

• Full Name: transmembrane 9 superfamily protein member 4
• Calculated Molecular Weight: 642 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC021107
• Gene Symbol: TM9SF4
• Gene ID (NCBI): 9777
• RRID: AB_2880149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92544
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncogene; Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.; Authors - Yifei Zhu ; KD Validated
mouse	WB,IF	Stem Cell Res Ther; TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes; Authors - Libo Yu ; KO Validated
human	IHC	J Exp Clin Cancer Res; CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells.; Authors - Ying Yu
mouse,human	WB,IHC,IF	Cell Mol Gastroenterol Hepatol; TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease; Authors - Mingxu Xie ; KD Validated
human,mouse	WB,IHC	Nat Commun; TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis; Authors - Zhaoyue Meng ; KD Validated