TM9SF4 Polyclonal antibody

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• TM9SF4 Polyclonal antibody (25595-1-AP)

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• KD/KO Validated

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25595-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

KIAA0255, TM9SF4

• 

  WB analysis using 25595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 25595-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A375 using 25595-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25595-1-AP (TM9SF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse colon using 25595-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25595-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A375 cells, A431 cells, HepG2 cells
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25595-1-AP targets TM9SF4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22335

  Product name: Recombinant human TM9SF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 25-244 aa of BC021107

  Sequence: IKAVKLTSSRTQLPYEYYSLPFCQPSKITYKAENLGEVLRGDRIVNTPFQVLMNSEKKCEVLCSQSNKPVTLTVEQSRLVAERITEDYYVHLIADNLPVATRLELYSNRDSDDKKKEKDVQFEHGYRLGFTDVNKIYLHNHLSFILYYHREDMEEDQEHTYRVVRFEVIPQSIRLEDLKADEKSSCTLPEGTNSSPQEIDPTKENQLYFTYSVHWEESDI

• Full Name: transmembrane 9 superfamily protein member 4
• Calculated Molecular Weight: 642 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC021107
• Gene Symbol: TM9SF4
• Gene ID (NCBI): 9777
• RRID: AB_2880149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92544
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The TM9SF4 antibody (Cat# 25595-1-AP) has 7 total citations across journals including Nature Communications, Oncogene, J Exp Clin Cancer Res, Cell Mol Gastroenterol Hepatol, Stem Cell Res Ther, Front Cell Infect Microbiol, and Discov Oncol. Research fields span tumor progression and metastasis, prostate cancer, chemoresistant breast cancer, inflammatory bowel disease, bone marrow mesenchymal stem cell lineage commitment, bacterial cytotoxicity in swine, and hepatocellular carcinoma prognosis.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis; Authors - Zhaoyue Meng ; KD Validated
human	IHC	J Exp Clin Cancer Res; CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells.; Authors - Ying Yu
human	WB,IHC	Oncogene; Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.; Authors - Yifei Zhu ; KD Validated
mouse,human	WB,IHC,IF	Cell Mol Gastroenterol Hepatol; TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease; Authors - Mingxu Xie ; KD Validated
mouse	WB,IF	Stem Cell Res Ther; TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes; Authors - Libo Yu ; KO Validated
pig	WB,IF	Front Cell Infect Microbiol; TM9SF4 acts as a receptor mediating Glaesserella parasuis cytolethal distending toxin-induced cytotoxicity in PK15 cells.; Authors - Li Lei ; KO Validated