TM9SF4 Polyclonal antibody

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29520-1-AP

Synonyms

dJ836N17.2, KIAA0255, TM9SF4

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Validation Data Gallery

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  WB analysis using 29520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29520-1-AP (TM9SF4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 29520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 29520-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 29520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29520-1-AP (TM9SF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A375 cells, PC-3 cells, mouse kidney tissue
• Positive IHC detected in: human stomach cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29520-1-AP targets TM9SF4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TM9SF4 fusion protein Ag31253
• Full Name: transmembrane 9 superfamily protein member 4
• Calculated Molecular Weight: 642 aa, 75 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC021107
• Gene Symbol: TM9SF4
• Gene ID (NCBI): 9777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.