TMCC1 Polyclonal antibody

TMCC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21099-1-AP

Synonyms

KIAA0779, TMCC1

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Validation Data Gallery

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  WB analysis using 21099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21099-1-AP (TMCC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 21099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21099-1-AP (TMCC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse embryo using 21099-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21099-1-AP (TMCC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HCT 116 cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse embryo tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21099-1-AP is TMCC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMCC1 fusion protein Ag15367
• Full Name: transmembrane and coiled-coil domain family 1
• Calculated Molecular Weight: 653 aa, 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC039859
• Gene Symbol: TMCC1
• Gene ID (NCBI): 23023
• RRID: AB_10733122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.