TMP21 Polyclonal antibody

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  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 15199-1-AP

  IP result of anti-TMP21 (IP:15199-1-AP, 4ug; Detection:15199-1-AP 1:3000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

TMP21 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15199-1-AP

Publications(9)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

TMED10, 21 kDa transmembrane-trafficking protein, p23, p24 family protein delta-1, p24delta1

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HEK-293T cells, Neuro-2a cells, PC-3 cells, Raji cells, U-87 MG cells, NIH/3T3 cells, mouse pancreas tissue, rat pancreas tissue
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: mouse brain tissue, human colon cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

15199-1-AP targets TMP21 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7347

  Product name: Recombinant human TMED10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 40-186 aa of BC001825

  Sequence: SRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRV

• Full Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001825
• Gene Symbol: TMED10
• Gene ID (NCBI): 10972
• RRID: AB_2204321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49755
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF	Cell; A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.; Authors - Min Zhang ; KO Validated
human	WB	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
human	WB	Brain Behav Immun; Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis; Authors - Mengya Jiao
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)

TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)

FH

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

• Applications: Immunofluorescence,