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  • KD/KO Validated

TMP21 Polyclonal antibody

TMP21 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15199-1-AP


P24(DELTA), p24delta, S31I125, S31III125, TMED10, Tmp 21 I, TMP21, Transmembrane protein Tmp21

Tested Applications

Positive WB detected inBxPC-3 cells, HEK-293T cells, Neuro-2a cells, PC-3 cells, Raji cells, U-87 MG cells, NIH/3T3 cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse brain tissue, human colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15199-1-AP targets TMP21 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMP21 fusion protein Ag7347
Full Name transmembrane emp24-like trafficking protein 10 (yeast)
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC001825
Gene Symbol TMED10
Gene ID (NCBI) 10972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for TMP21 antibody 15199-1-APDownload protocol
IHC protocol for TMP21 antibody 15199-1-APDownload protocol
IF protocol for TMP21 antibody 15199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li

J Cell Sci

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Authors - Jiaxin Sun

BMC Cancer

The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.

Authors - Xue Yang

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville

Adv Sci (Weinh)

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Authors - Hak Park


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)
TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

  • Applications: Immunofluorescence,