TMED2 Polyclonal antibody

TMED2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11981-1-AP

Synonyms

Membrane protein p24A, P24A, RNP24, TMED2

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Validation Data Gallery

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  WB analysis using 11981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11981-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 11981-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11981-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11981-1-AP (TMED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11981-1-AP (TMED2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11981-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11981-1-AP (TMED2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse pancreas tissue, mouse skin tissue, mouse liver tissue, HeLa cells, rat kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11981-1-AP targets TMED2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMED2 fusion protein Ag2607
• Full Name: transmembrane emp24 domain trafficking protein 2
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC025957
• Gene Symbol: TMED2
• Gene ID (NCBI): 10959
• RRID: AB_2204353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
human	WB	BMC Biol; ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.; Authors - Xihua Yue
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang
human	WB	Adv Sci (Weinh); TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.; Authors - Hak Park
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated
human	WB	Curr Med Sci; TMED2 Induces Cisplatin Resistance in Breast Cancer via Targeting the KEAP1-Nrf2 Pathway; Authors - Chen Liang